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| | ===Meaning=== | | ===Meaning=== |
| | Investigate the effect of [[pH]] on the rate of reaction for an [[enzyme]]. | | Investigate the effect of [[pH]] on the rate of reaction for an [[enzyme]]. |
| − | ===Method===
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| − | *[[Dependent Variable]] - The antiseptic or antibiotic used.
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| − | *[[Independent Variable]] - Observe the growth of colonies.
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| − | *[[Control Variable]]s - The same quantity of antiseptic and antibiotic. The same spread of [[bacteria]] applied to the growth medium. Incubated for the same length of time. Incubated at the same temperature. Only one type of [[bacteria]] used.
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| − | #Agar plates prepared with [[bacteria]] will be supplied.
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| − | #Mark the underside of an agar plate into three sections making a dot in the centre of each, labeling them 1,2 and 3 and writing your initials and the date on the agar plate.
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| − | #Spray the work space with disinfectant and wash your hands with antibacterial soap.
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| − | #Take a small circle of filter paper and soak it into one of the three antiseptics/antibiotics.
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| − | #Place the circle onto the dot marked at the centre of a section.
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| − | #Repeat steps 4 and 5 ensuring the antiseptics are not mixed in any way.
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| − | #Use two pieces of sellotape to attach the lid loosely to the top of the agar plate (to ensure oxygen get in).
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| − | #Incubate at 25°C for 48 hours.
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| − | #Measure the diameter of the area in which [[bacteria]] did not grow.
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Revision as of 20:16, 21 November 2018
Key Stage 4
Meaning
Investigate the effect of pH on the rate of reaction for an enzyme.