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Difference between revisions of "GCSE Chemistry Required Practical: Calculating Retention Factors"

(Created page with "==Key Stage 4== ===Meaning=== Use a Chromatography experiment to produce a Chromatogram and calculate the Retention Factors (R<sub>f</sub>) fo...")
 
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===Method===
 
===Method===
{| class="wikitable"
+
#Take a piece of [[chromatography]] paper of [[width]] 6cm and [[height]] 10cm.
|-
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#Using a ruler draw a line with pencil across the [[width]] 2cm up from the end.
|[[File:ChromatographyDiagram.png|center|500px]]
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#Add a dot of each unknown sample on the pencil line at 1cm [[interval]]s.
|}
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#Suspend the [[chromatography]] paper in a suitable [[solvent]] just below the pencil line with most of the paper above the [[solvent]].
 +
#Wait until the [[solvent]] stops rising up the paper.
 +
#Use a ruler to measure the distance that the [[solvent]] has traveled up the paper.
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#Use a ruler to measure the distance that the different [[dye]]s have traveled up the paper.
 +
#Use the following equation to find the [[Retention Factor]] for each [[dye]]:
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:<math>R_f = \frac{d_c}{d_s}</math>
 +
:Where:
 +
:R<sub>f</sub> = [[Retention Factor]]
 +
:d<sub>c</sub> = distance moved by the [[chemical]]
 +
:d<sub>s</sub> = distance moved by the [[solvent]]

Revision as of 11:36, 29 January 2019

Key Stage 4

Meaning

Use a Chromatography experiment to produce a Chromatogram and calculate the Retention Factors (Rf) for they dyes found within different inks.

Method

  1. Take a piece of chromatography paper of width 6cm and height 10cm.
  2. Using a ruler draw a line with pencil across the width 2cm up from the end.
  3. Add a dot of each unknown sample on the pencil line at 1cm intervals.
  4. Suspend the chromatography paper in a suitable solvent just below the pencil line with most of the paper above the solvent.
  5. Wait until the solvent stops rising up the paper.
  6. Use a ruler to measure the distance that the solvent has traveled up the paper.
  7. Use a ruler to measure the distance that the different dyes have traveled up the paper.
  8. Use the following equation to find the Retention Factor for each dye:

\[R_f = \frac{d_c}{d_s}\]

Where:
Rf = Retention Factor
dc = distance moved by the chemical
ds = distance moved by the solvent